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Alternatively, the stock tradition organisms could possibly be developed in an acceptable liquid medium (i.e., SoybeanCasein Digest Broth or Sabouraud Dextrose Broth) plus the cells harvested by centrifugation, then washed and resuspended in sterile saline TS to acquire a microbial count of about 1 × 108 cfu per mL. [Be awareThe estima